Journal: Immunologic Research
Article Title: Expression of JAK3, STAT2, STAT4, and STAT6 in pemphigus vulgaris
doi: 10.1007/s12026-020-09122-y
Figure Lengend Snippet: Morphometric analysis of JAK3 (upper left), STAT2 (upper right), STAT4 (lower left), and STAT6 (lower right) immunoexpression in keratinocytes. The results of semiquantitative analysis are expressed as the mean ± standard deviation. Control – normal skin. PV-P – pemphigus vulgaris perilesional skin, PV-L – pemphigus vulgaris skin lesions. The level of significance is defined where p < 0.05. JAK3: C vs. PV-L ( p < 0.05); PV-P vs. PV-L ( p < 0.05); STAT2: C vs. PV-L ( p < 0.05); STAT4: C vs. PV-P (NS), C vs. PV-L (NS); STAT6: C vs. PV-P ( p < 0.05), C vs. PV-L ( p < 0.05), PV-P vs. PV-L ( p < 0.05)
Article Snippet: Endogenous peroxidase activity was blocked by 0.3% hydrogen peroxide in distilled water and then, sections were rinsed with Tris-buffered saline (TBS, Dako, Denmark), and incubated with primary rabbit polyclonal antibody against STAT2 (Santa Cruz Biotechnology Inc.), mouse monoclonal antibody against STAT4 (Santa Cruz Biotechnology, Inc.), and primary rabbit polyclonal antibody against STAT6 (Santa Cruz biotechnology Inc.) and incubated overnight with mouse monoclonal antibody against JAK3.
Techniques: Standard Deviation, Control